Anthropology and Human Genetics
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prime-seq

Bulk RNA-seq has emerged as the standard tool to measure gene expression across many samples and conditions. However, the most widely used techniques are still rather expensive and time consuming, limiting the number of samples that can be done in a single experiment. While the field of single cell RNA-seq has exploded in recent years, many questions can still be answered by bulk RNA sequencing in a more reproducible, efficient and powerful way.

We systematically improved a bulk version of the single cell RNA-seq protocol SCRB-seq to increase throughput and further decrease costs. Using SPRI beads we can isolate RNA as part of the protocol enabling us to start from lysates instead of purified RNA. This eliminates one of the most time and cost intensive steps - namely RNA isolation using columns or phenol chloroform extraction - thereby increasing the throughput vastly.

bulk_seq_workflow

Links:
https://www.protocols.io/view/prime-seq-s9veh66

Research:

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https://doi.org/10.18632/oncotarget.21877
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https://doi.org/10.1101/2021.02.05.429919
https://doi.org/10.1101/2021.02.03.429351